SrasV1, Main, Exploration, bibRecord, 006079

Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus

Identifieur interne : 006079 ( Main/Exploration ); précédent : 006078; suivant : 006080

Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus

Auteurs : Jingqiang Wang ; Jie Wen ; Jingxiang Li ; Jianning Yin ; Qingyu Zhu ; Hao Wang ; Yongkui Yang ; E E Qin ; Bo You ; Wei Li ; Xiaolei Li ; Shengyong Huang ; Ruifu Yang ; Xumin Zhang ; Ling Yang ; Ting Zhang ; Ye Yin ; Xiaodai Cui ; Xiangjun Tang ; Luoping Wang ; Bo He ; Lianhua Ma ; Tingting Lei ; Changqing Zeng ; Jianqiu Fang ; Jun Yu ; Jian Wang ; Huanming Yang ; Matthew B. West ; Aruni Bhatnagar ; Youyong Lu ; Ningzhi Xu ; Siqi Liu

Source :

Clinical Chemistry [ 0009-9147 ] ; 2003.

RBID : PMC:7108193

Descripteurs français

KwdFr :
- Animaux, Anticorps antiviraux (sang), Cartographie épitopique (), Cellules Vero, Fragments peptidiques (immunologie), Fragments peptidiques (sang), Humains, Protéines virales structurales (), Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (sang), Syndrome respiratoire aigu sévère (virologie), Test ELISA, Tests sérologiques, Virus du SRAS (), Virus du SRAS (génétique).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Virus du SRAS.
- immunologie : Fragments peptidiques.
- sang : Anticorps antiviraux, Fragments peptidiques, Syndrome respiratoire aigu sévère.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cartographie épitopique, Cellules Vero, Humains, Protéines virales structurales, Test ELISA, Tests sérologiques, Virus du SRAS.

English descriptors

KwdEn :
- Animals, Antibodies, Viral (blood), Chlorocebus aethiops, Enzyme-Linked Immunosorbent Assay, Epitope Mapping (methods), Humans, Peptide Fragments (blood), Peptide Fragments (immunology), SARS Virus (chemistry), SARS Virus (genetics), Serologic Tests, Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology), Vero Cells, Viral Structural Proteins (chemistry).
MESH :
- chemical , blood : Antibodies, Viral, Peptide Fragments.
- chemical , chemistry : Viral Structural Proteins.
- chemical , immunology : Peptide Fragments.
- blood : Severe Acute Respiratory Syndrome.
- chemistry : SARS Virus.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- methods : Epitope Mapping.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Enzyme-Linked Immunosorbent Assay, Humans, Serologic Tests, Vero Cells.

Abstract

Background: The widespread threat of severe acute respiratory syndrome (SARS) to human life has spawned challenges to develop fast and accurate analytical methods for its early diagnosis and to create a safe antiviral vaccine for preventive use. Consequently, we thoroughly investigated the immunoreactivities with patient sera of a series of synthesized peptides from SARS-coronavirus structural proteins.

Methods: We synthesized 41 peptides ranging in size from 16 to 25 amino acid residues of relatively high hydrophilicity. The immunoreactivities of the peptides with SARS patient sera were determined by ELISA.

Results: Four epitopic sites, S599, M137, N66, and N371-404, located in the SARS-coronavirus S, M, and N proteins, respectively, were detected by screening synthesized peptides. Notably, N371 and N385, located at the COOH terminus of the N protein, inhibited binding of antibodies to SARS-coronavirus lysate and bound to antibodies in >94% of samples from SARS study patients. N385 had the highest affinity for forming peptide-antibody complexes with SARS serum.

Conclusions: Five peptides from SARS structural proteins, especially two from the COOH terminus of the N protein, appear to be highly immunogenic and may be useful for serologic assays. The identification of these antigenic peptides contributes to the understanding of the immunogenicity and persistence of SARS coronavirus.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108193

DOI: 10.1373/clinchem.2003.023184
PubMed: 14633869
PubMed Central: 7108193

Affiliations:

Le document en format XML

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<author><name sortKey="Wang, Luoping" sort="Wang, Luoping" uniqKey="Wang L" first="Luoping" last="Wang">Luoping Wang</name>
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<affiliation><nlm:aff>NONE</nlm:aff>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus</title>
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<affiliation><nlm:aff>NONE</nlm:aff>
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<author><name sortKey="Yin, Jianning" sort="Yin, Jianning" uniqKey="Yin J" first="Jianning" last="Yin">Jianning Yin</name>
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<affiliation><nlm:aff>NONE</nlm:aff>
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<author><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Hao" sort="Wang, Hao" uniqKey="Wang H" first="Hao" last="Wang">Hao Wang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
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<affiliation><nlm:aff>NONE</nlm:aff>
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<author><name sortKey="Yang, Yongkui" sort="Yang, Yongkui" uniqKey="Yang Y" first="Yongkui" last="Yang">Yongkui Yang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
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<author><name sortKey="Qin, E E" sort="Qin, E E" uniqKey="Qin E" first="E E" last="Qin">E E Qin</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="You, Bo" sort="You, Bo" uniqKey="You B" first="Bo" last="You">Bo You</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
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<author><name sortKey="Li, Xiaolei" sort="Li, Xiaolei" uniqKey="Li X" first="Xiaolei" last="Li">Xiaolei Li</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Huang, Shengyong" sort="Huang, Shengyong" uniqKey="Huang S" first="Shengyong" last="Huang">Shengyong Huang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Xumin" sort="Zhang, Xumin" uniqKey="Zhang X" first="Xumin" last="Zhang">Xumin Zhang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yang, Ling" sort="Yang, Ling" uniqKey="Yang L" first="Ling" last="Yang">Ling Yang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Ting" sort="Zhang, Ting" uniqKey="Zhang T" first="Ting" last="Zhang">Ting Zhang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yin, Ye" sort="Yin, Ye" uniqKey="Yin Y" first="Ye" last="Yin">Ye Yin</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Cui, Xiaodai" sort="Cui, Xiaodai" uniqKey="Cui X" first="Xiaodai" last="Cui">Xiaodai Cui</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Tang, Xiangjun" sort="Tang, Xiangjun" uniqKey="Tang X" first="Xiangjun" last="Tang">Xiangjun Tang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Luoping" sort="Wang, Luoping" uniqKey="Wang L" first="Luoping" last="Wang">Luoping Wang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="He, Bo" sort="He, Bo" uniqKey="He B" first="Bo" last="He">Bo He</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ma, Lianhua" sort="Ma, Lianhua" uniqKey="Ma L" first="Lianhua" last="Ma">Lianhua Ma</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lei, Tingting" sort="Lei, Tingting" uniqKey="Lei T" first="Tingting" last="Lei">Tingting Lei</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zeng, Changqing" sort="Zeng, Changqing" uniqKey="Zeng C" first="Changqing" last="Zeng">Changqing Zeng</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fang, Jianqiu" sort="Fang, Jianqiu" uniqKey="Fang J" first="Jianqiu" last="Fang">Jianqiu Fang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yu, Jun" sort="Yu, Jun" uniqKey="Yu J" first="Jun" last="Yu">Jun Yu</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="West, Matthew B" sort="West, Matthew B" uniqKey="West M" first="Matthew B" last="West">Matthew B. West</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bhatnagar, Aruni" sort="Bhatnagar, Aruni" uniqKey="Bhatnagar A" first="Aruni" last="Bhatnagar">Aruni Bhatnagar</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lu, Youyong" sort="Lu, Youyong" uniqKey="Lu Y" first="Youyong" last="Lu">Youyong Lu</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xu, Ningzhi" sort="Xu, Ningzhi" uniqKey="Xu N" first="Ningzhi" last="Xu">Ningzhi Xu</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Siqi" sort="Liu, Siqi" uniqKey="Liu S" first="Siqi" last="Liu">Siqi Liu</name>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
<affiliation><nlm:aff>NONE</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Clinical Chemistry</title>
<idno type="ISSN">0009-9147</idno>
<idno type="eISSN">1530-8561</idno>
<imprint><date when="2003">2003</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Viral (blood)</term>
<term>Chlorocebus aethiops</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Epitope Mapping (methods)</term>
<term>Humans</term>
<term>Peptide Fragments (blood)</term>
<term>Peptide Fragments (immunology)</term>
<term>SARS Virus (chemistry)</term>
<term>SARS Virus (genetics)</term>
<term>Serologic Tests</term>
<term>Severe Acute Respiratory Syndrome (blood)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Vero Cells</term>
<term>Viral Structural Proteins (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (sang)</term>
<term>Cartographie épitopique ()</term>
<term>Cellules Vero</term>
<term>Fragments peptidiques (immunologie)</term>
<term>Fragments peptidiques (sang)</term>
<term>Humains</term>
<term>Protéines virales structurales ()</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (sang)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Test ELISA</term>
<term>Tests sérologiques</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
<term>Peptide Fragments</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Structural Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Peptide Fragments</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Fragments peptidiques</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Epitope Mapping</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Fragments peptidiques</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Serologic Tests</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cartographie épitopique</term>
<term>Cellules Vero</term>
<term>Humains</term>
<term>Protéines virales structurales</term>
<term>Test ELISA</term>
<term>Tests sérologiques</term>
<term>Virus du SRAS</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p><italic>Background:</italic>
 The widespread threat of severe acute respiratory syndrome (SARS) to human life has spawned challenges to develop fast and accurate analytical methods for its early diagnosis and to create a safe antiviral vaccine for preventive use. Consequently, we thoroughly investigated the immunoreactivities with patient sera of a series of synthesized peptides from SARS-coronavirus structural proteins.</p>
<p><italic>Methods:</italic>
 We synthesized 41 peptides ranging in size from 16 to 25 amino acid residues of relatively high hydrophilicity. The immunoreactivities of the peptides with SARS patient sera were determined by ELISA.</p>
<p><italic>Results:</italic>
 Four epitopic sites, S599, M137, N66, and N371-404, located in the SARS-coronavirus S, M, and N proteins, respectively, were detected by screening synthesized peptides. Notably, N371 and N385, located at the COOH terminus of the N protein, inhibited binding of antibodies to SARS-coronavirus lysate and bound to antibodies in >94% of samples from SARS study patients. N385 had the highest affinity for forming peptide-antibody complexes with SARS serum.</p>
<p><italic>Conclusions:</italic>
 Five peptides from SARS structural proteins, especially two from the COOH terminus of the N protein, appear to be highly immunogenic and may be useful for serologic assays. The identification of these antigenic peptides contributes to the understanding of the immunogenicity and persistence of SARS coronavirus.</p>
</div>
</front>
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</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Bhatnagar, Aruni" sort="Bhatnagar, Aruni" uniqKey="Bhatnagar A" first="Aruni" last="Bhatnagar">Aruni Bhatnagar</name>
<name sortKey="Cui, Xiaodai" sort="Cui, Xiaodai" uniqKey="Cui X" first="Xiaodai" last="Cui">Xiaodai Cui</name>
<name sortKey="Fang, Jianqiu" sort="Fang, Jianqiu" uniqKey="Fang J" first="Jianqiu" last="Fang">Jianqiu Fang</name>
<name sortKey="He, Bo" sort="He, Bo" uniqKey="He B" first="Bo" last="He">Bo He</name>
<name sortKey="Huang, Shengyong" sort="Huang, Shengyong" uniqKey="Huang S" first="Shengyong" last="Huang">Shengyong Huang</name>
<name sortKey="Lei, Tingting" sort="Lei, Tingting" uniqKey="Lei T" first="Tingting" last="Lei">Tingting Lei</name>
<name sortKey="Li, Jingxiang" sort="Li, Jingxiang" uniqKey="Li J" first="Jingxiang" last="Li">Jingxiang Li</name>
<name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<name sortKey="Li, Xiaolei" sort="Li, Xiaolei" uniqKey="Li X" first="Xiaolei" last="Li">Xiaolei Li</name>
<name sortKey="Liu, Siqi" sort="Liu, Siqi" uniqKey="Liu S" first="Siqi" last="Liu">Siqi Liu</name>
<name sortKey="Lu, Youyong" sort="Lu, Youyong" uniqKey="Lu Y" first="Youyong" last="Lu">Youyong Lu</name>
<name sortKey="Ma, Lianhua" sort="Ma, Lianhua" uniqKey="Ma L" first="Lianhua" last="Ma">Lianhua Ma</name>
<name sortKey="Qin, E E" sort="Qin, E E" uniqKey="Qin E" first="E E" last="Qin">E E Qin</name>
<name sortKey="Tang, Xiangjun" sort="Tang, Xiangjun" uniqKey="Tang X" first="Xiangjun" last="Tang">Xiangjun Tang</name>
<name sortKey="Wang, Hao" sort="Wang, Hao" uniqKey="Wang H" first="Hao" last="Wang">Hao Wang</name>
<name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
<name sortKey="Wang, Jingqiang" sort="Wang, Jingqiang" uniqKey="Wang J" first="Jingqiang" last="Wang">Jingqiang Wang</name>
<name sortKey="Wang, Luoping" sort="Wang, Luoping" uniqKey="Wang L" first="Luoping" last="Wang">Luoping Wang</name>
<name sortKey="Wen, Jie" sort="Wen, Jie" uniqKey="Wen J" first="Jie" last="Wen">Jie Wen</name>
<name sortKey="West, Matthew B" sort="West, Matthew B" uniqKey="West M" first="Matthew B" last="West">Matthew B. West</name>
<name sortKey="Xu, Ningzhi" sort="Xu, Ningzhi" uniqKey="Xu N" first="Ningzhi" last="Xu">Ningzhi Xu</name>
<name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name>
<name sortKey="Yang, Ling" sort="Yang, Ling" uniqKey="Yang L" first="Ling" last="Yang">Ling Yang</name>
<name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name>
<name sortKey="Yang, Yongkui" sort="Yang, Yongkui" uniqKey="Yang Y" first="Yongkui" last="Yang">Yongkui Yang</name>
<name sortKey="Yin, Jianning" sort="Yin, Jianning" uniqKey="Yin J" first="Jianning" last="Yin">Jianning Yin</name>
<name sortKey="Yin, Ye" sort="Yin, Ye" uniqKey="Yin Y" first="Ye" last="Yin">Ye Yin</name>
<name sortKey="You, Bo" sort="You, Bo" uniqKey="You B" first="Bo" last="You">Bo You</name>
<name sortKey="Yu, Jun" sort="Yu, Jun" uniqKey="Yu J" first="Jun" last="Yu">Jun Yu</name>
<name sortKey="Zeng, Changqing" sort="Zeng, Changqing" uniqKey="Zeng C" first="Changqing" last="Zeng">Changqing Zeng</name>
<name sortKey="Zhang, Ting" sort="Zhang, Ting" uniqKey="Zhang T" first="Ting" last="Zhang">Ting Zhang</name>
<name sortKey="Zhang, Xumin" sort="Zhang, Xumin" uniqKey="Zhang X" first="Xumin" last="Zhang">Xumin Zhang</name>
<name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name>
</noCountry>
</tree>
</affiliations>
</record>

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Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus

Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome Coronavirus

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